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KMID : 0616120110420040348
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Korean Journal of Pharmacognosy
2011 Volume.42 No. 4 p.348 ~ p.353
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The Effects of Dictamni Radicis Cortex on the iNOS Expression and Proinflammatory Cytokines Production
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Park Jeong-Suk
Shin Tae-Yong
Kim Dae-Keun
Lee Jae-Hyeok
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Abstract
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The aim of the present study is to investigate the cytokine production inhibitory effect of a Dictamni Radicis Cortex (DRC). DRC has been commonly used as important medicinal herb in China and it used to control eczema, atopic dermatitis, fever and inflammatory diseases. Inflammation, such as a bacterial infection in vivo metabolites, such as external stimuli or internal stimuli to the defense mechanisms of the biological tissue a variety of intracellular regulatory factors deulin inflammatory TNF-α, IL-1β, IL-6, IL-8, such as proinflammatory cytokines, prostagrandin, lysosomal enzyme, free radicals are involved in a variety of mediators. The present study was designed to determine the effect of the DRC on proinflammatory factors such as NO, iNOS expression and TNF-α, IL-1β, IL-6 in lipopolysaccharide (LPS) - stimulated RAW264.7 cells. The cell toxicity was determined by MTS assay. To evaluate of anti-inflammatory effect of DRC, amount of NO was measured using the NO detection kit and the iNOS expression was measured by reverse transcriptase polymerase chain reaction (RT-PCR). And proinflammatory cytokines were measured by ELISA kit. As a result, the DRC reduced NO, iNOS expression and TNF-α, IL-1β, IL-6 production without cytotoxicity. Our results suggest that the DRC may have an anti-inflammatory property through suppressing inflammatory mediator productions.
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KEYWORD
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Dictamni Radicis Cortex, Cytokine, NO, iNOS, TNF-α
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